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- Prestained Protein Ladder (11 – 270 kDa)
(HB9198)
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Product overview
| Name | Prestained Protein Ladder (11 – 270 kDa) |
| Biological description | Dual color protein broad range protein ladder used for estimated the molecular weight of unknown proteins in SDS-PAGE and Western Blotting. Contains 10 proteins of known molecular weights coupled to chromophores for easy visualisation. |
| Description | Dual color broad range protein ladder |
Biological Data Related Areas Calculators Technical guides (1) Reviews References (1)
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Images
Prestained Protein Ladder (11 – 270 kDa)
5µL loaded onto a 4-20% Tris-glycine SDS-PAGE gel and run at 160V for approximately 60 minutes before being transferred to a PVDF membrane for 90 minutes at 400mA.
Comparison between Prestained Protein Ladder (11 – 270 kDa) and NEB P7718S.
5µL each of HB9198 and NEB P7718S were loaded onto a 4-20% Tris-glycine SDS-PAGE gel and run at 160V for approximately 60 minutes before being transferred to a PVDF membrane for 90 minutes at 400mA.
Prestained Protein Ladder (11 – 270 kDa)
5µL loaded onto a 4-20% Tris-glycine SDS-PAGE gel and run at 160V for approximately 60 minutes before being transferred to a PVDF membrane for 90 minutes at 400mA.
Comparison between Prestained Protein Ladder (11 – 270 kDa) and NEB P7718S.
5µL each of HB9198 and NEB P7718S were loaded onto a 4-20% Tris-glycine SDS-PAGE gel and run at 160V for approximately 60 minutes before being transferred to a PVDF membrane for 90 minutes at 400mA.
Biological Data
| Application notes | We recommend loading between 2µl and 5µl per lane of a polyacrylamide gel. There is no need heat, reduce or dilute in sample buffer before use. |
Solubility & Handling
| Storage instructions | -20°C |
| Storage buffer | 62.5 mM Tris-H3PO4 (pH 7.5), 2% SDS, 1 mM EDTA, 10 mM DTT, 1 mM sodium azide, 33% glycerol |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Calculators
Molarity
More Info
Dilution
More Info
Related areas
- Type
Technical guides
How to Choose a Loading Control for Western Blots
References for Prestained Protein Ladder (11 – 270 kDa)
References are publications that support the biological activity of the product
-
Western blot: technique, theory, and trouble shooting.
Mahmood T et al (2012) North American journal of medical sciences 4 : 429-34
1 Item
Related Products
Dual color broad range protein ladder
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